Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. 304 signal level saturation. Molecular Device SpectraMax M5 Plate Reader - Certified with Warranty. Wavelength optimization. Description 1. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The following document is intended to demonstrate setup of this instrument. 2 mm long, 86. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. NORTH AMERICA. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. 3: The cuvette chamber. . For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 26 5039228D Note. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 314 low lamp level warning. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. spr * SpectraMax i3(x) SpectraTest ABS2 v1. 01) + 0. Three kits covering a wide range of dsDNA concentrations. SoftMax Pro. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. The plate reader. The integration time suggested is 5-10 sec. . Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. These all-inclusive assay kits are ready to use in assay validation or screening. The excitation and the emission wavelengths on. 5. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The dual monochromator optics allow the widest range of applications to be utilized for bior. We would like to show you a description here but the site won’t allow us. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. Features. 5. Description 1. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 1. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. 3. Samples were analyzed in eight to sixteen wells per independent. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. you can use a resource. SpectraMax Microplate Readers. With optimized reagents, option to operate the. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Fluorescence Microplate Reader Comparison Chart. Cell-based experiments In the first experiment, we prepared a dilution series of CHO-K1 cells transiently transfected withThe SpectraMax 190 reader is the tool for DNA analysis. The dual monochromator optics allow. These robust readers have been placed in labs from Antarctica to the. edu) to be added as a trained user in iLab. The cells were then washed and staining quantitation was performed using a fluorescent plate reader (SpectraMax M5 Molecular Devices, Sunnyvale, CA) at 492/520 excitation emission wavelengths. Published Dec 16, 2020 | Updated Dec 16, 2020. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. SoftMax Pro 5 GxP Software provides all the data collection and analysis tools of the standard edition, along withMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit The Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. The PathCheck sensor can extend the instrument linearity by a factor of three or more. Fluorescence Microplate Reader Comparison Chart. We would like to show you a description here but the site won’t allow us. ) Click the "OK" button on SoftMax Pro Setup dialog box . Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. . Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax M5, Analyst HT or Analyst GT. The following document is intended to demonstrate setup of this instrument and provide representativeUsed together with the SpectraMax iD3 reader, the EarlyTox Cell Viability Assay Kits enable direct measurement of live and dead or apoptotic cells with a simple workflow and the increased throughput offered by a microplate format. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Lmicroplate luminometer. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Monday to Friday 7am - 5pm Pacific Time. The . Highly selective for dsDNA over ssDNA or RNA. Small, unbound molecules rotate rapidly in solution (top), while large,. These robust readers have been placed in labs from. 3. Plate chamber design of the SpectraMax category 1 microplate readers. spr SpectraMax Plus 384. . Table of Contents. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. 2. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Softmax Pro GxP Software 6. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. . To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. Select the fluorescence option and read the total fluorescence. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 5. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Control Panel The control panel houses the instrument status LEDs and the microplate. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. 72 MB. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. 304 signal level saturation. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Select the fluorescence option and read the total fluorescence. 9000-0756. The following document is intended to demonstrate setup of this instrument. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 303 unable to cal dark current. free tracer decreases, resulting in an. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. SpectraMax Quant dsDNA Assay Kits. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). 72 MB. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. 306 plate air cal fail,low light. Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. . 1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 6. 7°C and the bottom to 36. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 00 USD. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. 315 can't find zero order. Description. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 0 out of 5 $ 15,999. The corrected absorbance is within 5% of the. . Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. The following document is intended to demonstrate setup of this. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Example: [Drawer]. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. • Tel. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. 314 low lamp level warning. Spectral Range. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. . com A. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . 313 reference gain check fail. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. the IMAP Platform and smoothly transition into high-throughput screening. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. . 00 USD. The following document is intended to demonstrate setup of this. 3: The cuvette chamber. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. D 1. The plate reader can measure multiple readouts, including UV. Table 1. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. . HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). These all-inclusive assay kits are ready to use in assay validation or screening. Wide range of applications. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Please Inquire. Time-Resolved Fluorescence (TRF) Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing. +44-118-944-8000. 3. 00 USD. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. used. The plate reader can measure multiple readouts, including UV and visible absorbance. Microplate Reader Shelf. . SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. Description 1. Molecular Devices SpectraMax 190 Microplate Absorbance Spectrophotometer. 4. Refurbished. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 19. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5 Multi-Mode Microplate Reader. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 2. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. In either case. 0 out of 5 $ 15,999. There were 12 replicate wells per dilution in both 96-well and 384- well plates. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. Wavelength Range. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. D 1. UV/Vis Photometric Performance:. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. 6 lbs. 190-1000 nm, tunable in 1 nm increments. Table of Contents. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. Description 1. Find regional support. . SpectraMax® Microplate Readers and SoftMax® Pro Software. Detection of pro-inflammatory factors. For example, microplates containing different samples can be read using theThe interval between reads was set at 15 s or 60 s, respectively. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Exhibit with us {{>currencyLabel}} Back. SOFTmax PRO UserÕs Manual iii Contents Figures . The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Examples: “Press carefully to engage,” “ Do not press down. The chamber contains springs that position the cuvette in the proper alignment for a read. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. . Add to Cart. . . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 1). 5. The REF button has no valid meaning for. The following document is intended to demonstrate setup of this. Description 1. Five preferred detection modes. . . MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. ScanLater Western Blot Detection Cartridge. UV/Vis absorbance and fluorescence spectra were measured on SpectraMax® M5 Multi- Mode Microplate Reader. SpectraMax Glo and DuoLuc. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. 190-1000 nm, tunable in 1 nm increments. 0 (GraphPad Software, La Jolla, CA, USA) and SPSS version 23. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. . The guide also covers the software features, the specifications, and the accessories of the systems. ISM Equipment Classification (Group 1, Class A) This equipment is. The chamber. Molecular Devices SpectraMax M5 Multimode Microplate Reader. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. , ~488 nm), has the optical. 1. The assay window spanned four orders of magnitude. Purchase Overlord with the required number of. . The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 3 or greater. Add to Cart. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Choose from a three- (M3), four- (M4), orThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Category 2 and 3 microplate readers use gradient heating as well. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Description 1. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataSpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. type Clear. SpectraMax DuoLuc Reporter Assay (SMP 6. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. The assay applies fluorescence polarization to quantify Fc-containing IgG. Data Acquisition & Analysis Software. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. For example, microplates containing different samples can be read using the312 gain calibration failed. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. Description 1. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Read fluorescence polarization at room temperature (RT, 19–25°C). 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Bookings are free, but you will need to use iLab to book. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. 0 mL). The dynamic range of the assay was approximately 5 orders of magnitude. 3. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 3. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Bookings are free, but you will need to use iLab to book. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 306 plate air cal. Softmax Pro GxP Software 6. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Note: In this user guide, all references to SpectraMax Multi-Mode. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Step 2. . The M5 can read volumes down to 2uL with the. 1. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Access this by going to Help >. For wavelengths in the UV range above 220 nm,. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. HTRF is a proprietary time-resolved fluorescence technology that overcomes The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. Add to Cart. D 1 1. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 3. When the user applies a set temperature of 37°C, the top is heated to 37. I have been using the SpectraMax M5 since 2011. 5. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 4. Flexible for 6-well to 384-well microplates. Methods Preparation of reagents and standards. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). When the user applies a set temperature of 37°C, the top is heated to 37. Multimode Reader Comparison Chart. Open the Softmax Pro software and select the FP option. . SpectraMax M5 device. . Description 1. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. . Cytokines in medium released. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 7°C (gradient heating design). Cytokines in medium released.